Proteomic Analysis of Seminal Plasma and Sperm for Identification of Fertility Biomarkers in Frisiean Holstein Bulls

22:00:00

 

Bogor

 

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Asking about research sample

1. Frozen-thawed semen is also possible to use as research sample. But the spermatozoa pellet need to be washed by cold PBS supplemented with protease inhibitors at least three times to revise the proteins in the cryoprotectants. 2. The number of replication for 2D-PAGE, three biological replications and three technical replications. For mass-spectrometry, one repeat is the minimum but three biological repeats are preferred. 3. The sum of sample about 10 bulls of the same breed with fertility data is recommended to generate quality data that could be published.

- Improve the scientific reading, writing and communication skills - Looking for the available laboratory that possible to do the research - Learning more about statistic

We discussed about the sample and the method of frozen-thawed spermatozoa protein extraction and about the replication data

 

- Revised your PowerPoint presentation (PPt), get guidance from Drs. Ligaya and Purwantara, revise, and send the revised ppt research proposal to Prof Erdogan

Virtual (Online)

 

 

 

Davin Lintang, Wahid Oki